Assembling actin filaments for protrusion

Actin Filaments

Dark Matter Substructure , Filaments and Assembling Disks

We review some general properties of assembling galactic dark matter (DM) halos which have a direct effect on the baryon dynamics. Specifically, we focus on the mutual dynamical feedback between baryons and DM which influence disk formation and evolution, by comparing models with and without baryons evolved from identical initial conditions. Baryons are found to be capable of modifying the DM d...

Rapid actin transport during cell protrusion.

Transformed rat fibroblasts expressing two variants of green fluorescent protein, each fused to beta-actin, were used to study actin dynamics during cell protrusion. The recently developed FLAP (fluorescence localization after photobleaching) method permits the tracking of one fluorophore after localized photobleaching by using the other as a colocalized reference. Here, by visualizing the rati...

Dynamic stabilization of actin filaments.

We report here that actin filaments in vitro exist in two populations with significantly different shrinkage rates. Newly polymerized filaments shrink rapidly, primarily from barbed ends, at 1.8/s, but as they age they switch to a stable state that shrinks slowly, primarily from pointed ends, at approximately 0.1/s. This dynamic filament stabilization runs opposite to the classical prediction t...

Actin Filaments as Tension Sensors

The field of mechanobiology has witnessed an explosive growth over the past several years as interest has greatly increased in understanding how mechanical forces are transduced by cells and how cells migrate, adhere and generate traction. Actin, a highly abundant and anomalously conserved protein, plays a large role in forming the dynamic cytoskeleton that is so essential for cell form, motili...